Recombinant:Article Title: A PD-1-targeted, receptor-masked IL-2 immunocytokine that engages IL-2Rα strengthens T cell-mediated anti-tumor therapies.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pGL4.52[luc2P/STAT5RE/Hygro] Vector Promega Cat# E4561 Human IgG expression vector Regeneron N/A Mouse IgG expression vector Regeneron N/A Software and algorithms FlowJo v10 BD Biosciences RRID:SCR_008520 GraphPad Prism 10 GraphPad Software RRID:SCR_002798 Cell Ranger (v6.1.2) 10x Genomics RRID:SCR_017344 Seurat v4 https://satijalab.org/seurat/ RRID:SCR_016341 scRepertoire (v1.13.0) https://bioconductor.org/packages/ scRepertoire https://doi.org/10.18129/B9.bioc. scRepertoire R The R project for Statistical Computing https://www.r-project.org/ Other gentleMACSTM C Tubes Miltenyi Biotec Cat# 130-096-334 MACS SmartStrainers (70 mm) Miltenyi Biotec Cat# 130-098-462 Corning 100mm Cell Strainer Corning Cat# 431752 Sensor Chip CM5 Cytiva Cat# BR100530 Cell Reports Medicine 5, 101747, October 15, 2024 e5 OPEN ACCESS MC38 (from National Cancer Institute Lab of Tumor Immunology and Biology), MC38.Ova (described before39), B16F10 (ATCC, CRL-6475), MCA205 (Sigma-Aldrich, SCC173) and TRAMP-C2 (ATCC, CRL-2731) tumor cells were cultured in DME (Irvine Scientific, 9031) media supplemented with 10% FBS (Seradigm, 1500-500) and 1X L-glutamine/Penicillin/Streptomycin (Gibco, 10378016). ..
Expressing:Article Title: A PD-1-targeted, receptor-masked IL-2 immunocytokine that engages IL-2Rα strengthens T cell-mediated anti-tumor therapies.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pGL4.52[luc2P/STAT5RE/Hygro] Vector Promega Cat# E4561 Human IgG expression vector Regeneron N/A Mouse IgG expression vector Regeneron N/A Software and algorithms FlowJo v10 BD Biosciences RRID:SCR_008520 GraphPad Prism 10 GraphPad Software RRID:SCR_002798 Cell Ranger (v6.1.2) 10x Genomics RRID:SCR_017344 Seurat v4 https://satijalab.org/seurat/ RRID:SCR_016341 scRepertoire (v1.13.0) https://bioconductor.org/packages/ scRepertoire https://doi.org/10.18129/B9.bioc. scRepertoire R The R project for Statistical Computing https://www.r-project.org/ Other gentleMACSTM C Tubes Miltenyi Biotec Cat# 130-096-334 MACS SmartStrainers (70 mm) Miltenyi Biotec Cat# 130-098-462 Corning 100mm Cell Strainer Corning Cat# 431752 Sensor Chip CM5 Cytiva Cat# BR100530 Cell Reports Medicine 5, 101747, October 15, 2024 e5 OPEN ACCESS MC38 (from National Cancer Institute Lab of Tumor Immunology and Biology), MC38.Ova (described before39), B16F10 (ATCC, CRL-6475), MCA205 (Sigma-Aldrich, SCC173) and TRAMP-C2 (ATCC, CRL-2731) tumor cells were cultured in DME (Irvine Scientific, 9031) media supplemented with 10% FBS (Seradigm, 1500-500) and 1X L-glutamine/Penicillin/Streptomycin (Gibco, 10378016). ..
Plasmid Preparation:Article Title: A PD-1-targeted, receptor-masked IL-2 immunocytokine that engages IL-2Rα strengthens T cell-mediated anti-tumor therapies.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pGL4.52[luc2P/STAT5RE/Hygro] Vector Promega Cat# E4561 Human IgG expression vector Regeneron N/A Mouse IgG expression vector Regeneron N/A Software and algorithms FlowJo v10 BD Biosciences RRID:SCR_008520 GraphPad Prism 10 GraphPad Software RRID:SCR_002798 Cell Ranger (v6.1.2) 10x Genomics RRID:SCR_017344 Seurat v4 https://satijalab.org/seurat/ RRID:SCR_016341 scRepertoire (v1.13.0) https://bioconductor.org/packages/ scRepertoire https://doi.org/10.18129/B9.bioc. scRepertoire R The R project for Statistical Computing https://www.r-project.org/ Other gentleMACSTM C Tubes Miltenyi Biotec Cat# 130-096-334 MACS SmartStrainers (70 mm) Miltenyi Biotec Cat# 130-098-462 Corning 100mm Cell Strainer Corning Cat# 431752 Sensor Chip CM5 Cytiva Cat# BR100530 Cell Reports Medicine 5, 101747, October 15, 2024 e5 OPEN ACCESS MC38 (from National Cancer Institute Lab of Tumor Immunology and Biology), MC38.Ova (described before39), B16F10 (ATCC, CRL-6475), MCA205 (Sigma-Aldrich, SCC173) and TRAMP-C2 (ATCC, CRL-2731) tumor cells were cultured in DME (Irvine Scientific, 9031) media supplemented with 10% FBS (Seradigm, 1500-500) and 1X L-glutamine/Penicillin/Streptomycin (Gibco, 10378016). ..
Software:Article Title: A PD-1-targeted, receptor-masked IL-2 immunocytokine that engages IL-2Rα strengthens T cell-mediated anti-tumor therapies.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pGL4.52[luc2P/STAT5RE/Hygro] Vector Promega Cat# E4561 Human IgG expression vector Regeneron N/A Mouse IgG expression vector Regeneron N/A Software and algorithms FlowJo v10 BD Biosciences RRID:SCR_008520 GraphPad Prism 10 GraphPad Software RRID:SCR_002798 Cell Ranger (v6.1.2) 10x Genomics RRID:SCR_017344 Seurat v4 https://satijalab.org/seurat/ RRID:SCR_016341 scRepertoire (v1.13.0) https://bioconductor.org/packages/ scRepertoire https://doi.org/10.18129/B9.bioc. scRepertoire R The R project for Statistical Computing https://www.r-project.org/ Other gentleMACSTM C Tubes Miltenyi Biotec Cat# 130-096-334 MACS SmartStrainers (70 mm) Miltenyi Biotec Cat# 130-098-462 Corning 100mm Cell Strainer Corning Cat# 431752 Sensor Chip CM5 Cytiva Cat# BR100530 Cell Reports Medicine 5, 101747, October 15, 2024 e5 OPEN ACCESS MC38 (from National Cancer Institute Lab of Tumor Immunology and Biology), MC38.Ova (described before39), B16F10 (ATCC, CRL-6475), MCA205 (Sigma-Aldrich, SCC173) and TRAMP-C2 (ATCC, CRL-2731) tumor cells were cultured in DME (Irvine Scientific, 9031) media supplemented with 10% FBS (Seradigm, 1500-500) and 1X L-glutamine/Penicillin/Streptomycin (Gibco, 10378016). ..
Magnetic Cell Separation:Article Title: A PD-1-targeted, receptor-masked IL-2 immunocytokine that engages IL-2Rα strengthens T cell-mediated anti-tumor therapies.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pGL4.52[luc2P/STAT5RE/Hygro] Vector Promega Cat# E4561 Human IgG expression vector Regeneron N/A Mouse IgG expression vector Regeneron N/A Software and algorithms FlowJo v10 BD Biosciences RRID:SCR_008520 GraphPad Prism 10 GraphPad Software RRID:SCR_002798 Cell Ranger (v6.1.2) 10x Genomics RRID:SCR_017344 Seurat v4 https://satijalab.org/seurat/ RRID:SCR_016341 scRepertoire (v1.13.0) https://bioconductor.org/packages/ scRepertoire https://doi.org/10.18129/B9.bioc. scRepertoire R The R project for Statistical Computing https://www.r-project.org/ Other gentleMACSTM C Tubes Miltenyi Biotec Cat# 130-096-334 MACS SmartStrainers (70 mm) Miltenyi Biotec Cat# 130-098-462 Corning 100mm Cell Strainer Corning Cat# 431752 Sensor Chip CM5 Cytiva Cat# BR100530 Cell Reports Medicine 5, 101747, October 15, 2024 e5 OPEN ACCESS MC38 (from National Cancer Institute Lab of Tumor Immunology and Biology), MC38.Ova (described before39), B16F10 (ATCC, CRL-6475), MCA205 (Sigma-Aldrich, SCC173) and TRAMP-C2 (ATCC, CRL-2731) tumor cells were cultured in DME (Irvine Scientific, 9031) media supplemented with 10% FBS (Seradigm, 1500-500) and 1X L-glutamine/Penicillin/Streptomycin (Gibco, 10378016). ..
Cell Culture:Article Title: A PD-1-targeted, receptor-masked IL-2 immunocytokine that engages IL-2Rα strengthens T cell-mediated anti-tumor therapies.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pGL4.52[luc2P/STAT5RE/Hygro] Vector Promega Cat# E4561 Human IgG expression vector Regeneron N/A Mouse IgG expression vector Regeneron N/A Software and algorithms FlowJo v10 BD Biosciences RRID:SCR_008520 GraphPad Prism 10 GraphPad Software RRID:SCR_002798 Cell Ranger (v6.1.2) 10x Genomics RRID:SCR_017344 Seurat v4 https://satijalab.org/seurat/ RRID:SCR_016341 scRepertoire (v1.13.0) https://bioconductor.org/packages/ scRepertoire https://doi.org/10.18129/B9.bioc. scRepertoire R The R project for Statistical Computing https://www.r-project.org/ Other gentleMACSTM C Tubes Miltenyi Biotec Cat# 130-096-334 MACS SmartStrainers (70 mm) Miltenyi Biotec Cat# 130-098-462 Corning 100mm Cell Strainer Corning Cat# 431752 Sensor Chip CM5 Cytiva Cat# BR100530 Cell Reports Medicine 5, 101747, October 15, 2024 e5 OPEN ACCESS MC38 (from National Cancer Institute Lab of Tumor Immunology and Biology), MC38.Ova (described before39), B16F10 (ATCC, CRL-6475), MCA205 (Sigma-Aldrich, SCC173) and TRAMP-C2 (ATCC, CRL-2731) tumor cells were cultured in DME (Irvine Scientific, 9031) media supplemented with 10% FBS (Seradigm, 1500-500) and 1X L-glutamine/Penicillin/Streptomycin (Gibco, 10378016). ..
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